double-stranded dna中文什么意思

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用"double-stranded dna"造句

雙鏈dna

double: adj. 1.兩倍的，加倍的。 2.雙的，二重的，雙重的 ...
strand: n. 1.(繩子的)股，絞；一股繩子；纖維，繩，線；串。 ...
dna: DNA ＝ 1.deoxyribonucle ...
antibodies to double stranded dna: 雙螺旋脫氧核糖核酸抗體
circular double stranded dna: 環(huán)狀雙鏈dna

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The cyanophage contained a double - stranded dna genome at ca . 36 . 6kb
病毒的遺傳物質(zhì)為雙鏈dna ，基因組的大小約36 . 6kb 。
The genetic material of the new virus is arranged in a similar fashion , encoded in circular , double - stranded dna , and the virus ' s five proteins have similarities to the proteins of other polyoma viruses
新病毒的遺傳物質(zhì)排列方式與多瘤病毒類似，編碼為環(huán)狀的雙鏈dna ，新病毒的五個(gè)蛋白質(zhì)與其他多瘤病毒有相似之處。
To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells . methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
方法：根據(jù)端粒酶htert基因1573 ? 1591位的核酸序列，構(gòu)建帶t7啟動(dòng)子的部分雙鏈dna模板，用t7rna聚合酶體外合成短鏈shrna 。
Conclusions : the in - vitro method that partial double - strand dna with t7 wi l . - toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield , excellent purity shrna . lt is a convenient - , effective ^ low - cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory
結(jié)論：以帶t7啟動(dòng)子部分雙鏈dna為模板，用t7rna聚合酶體外合成出的shrna產(chǎn)量較高，純度較好，是一種簡便、高效、低成本的短鏈rna的制備方法，適合于普通實(shí)驗(yàn)室用來進(jìn)行短鏈rna的合成和rna干擾實(shí)驗(yàn)。
The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action , thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained . in this study , we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template . the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究設(shè)計(jì)以c tamrna為模板的反義cdna片段，從c ta基因5 ’端第94位到500位核苷酸段設(shè)計(jì)引物，目的片段407bp ，覆蓋第116和188位兩個(gè)aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點(diǎn)：用常規(guī)分子生物學(xué)方法構(gòu)建了反義片段的腺病毒表達(dá)載體( padeasy - 1系統(tǒng)) ；腺病毒載體經(jīng)hek293細(xì)胞包裝產(chǎn)生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進(jìn)行體外基因轉(zhuǎn)移，分別用反義片段真核表達(dá)載體轉(zhuǎn)染p388d1細(xì)胞和用重組腺病毒感染hela細(xì)胞，觀察導(dǎo)入的c ta基因反義rna抑制細(xì)胞內(nèi)組成型或誘導(dǎo)型c ta基因表達(dá)的作用，從而達(dá)到調(diào)控mhc -類分子表達(dá)的目的。